FRIDAY January 31, 2020
8:00 – 8:30 Registration/Coffee and Light Breakfast (Provided)
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8:30 – 9:00 Welcoming and Opening Remarks
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Conference Opening: Janet A. W. Elliott
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Welcome: Deborah James, Associate Vice-President (Innovation), University of Alberta
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9:00 – 10:00 Keynote Session 1: Normothermic Organ Perfusion: Dovetailing with Cryobiology
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Darren Freed
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Department of Surgery, University of Alberta, Edmonton, AB, Canada
10:00 – 10:20 A low-cost method of rat ex vivo lung perfusion for cryopreservation
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Larissa Lautner1, Jason P. Acker2,3, Jayan Nagendran1,4
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1Department of Surgery, University of Alberta, Edmonton, AB, Canada
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2Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
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3Centre for Innovation, Canadian Blood Services, Edmonton, AB, Canada
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4Mazankowski Alberta Heart Institute, University of Alberta, Edmonton, AB, Canada
10:20 – 10:40 Review of strategies to optimize cryopreservation of articular cartilage
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Kezhou Wu1,Leila Laouar1, Janet A. W. Elliott2,3, Nadr M. Jomha1
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1Division of Orthopedic Surgery, Department of Surgery, University of Alberta, Edmonton, AB, Canada
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2Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada
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3Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
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10:40 – 11:00 Coffee Break (Provided)
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11:00 – 11:20 Time Dependent Osmotic Damage in Sea Urchin Oocytes
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Dominic Olver1 Pablo Heres2, Estefania Paredes2, James D Benson1
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1Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada
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2Department of Ecology and Marine Biology & Marine Biological Resources Functional Preservation Service at ECIMAT marine Station, University of Vigo, Vigo, Spain
11:20 – 11:40 The Effect of Length of Storage of Cryoprotectant Solutions on Chondrocyte Cell Viability: Study Development and Preliminary Findings
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Johnathan Sevick1,2 Kezhou Wu2, Leila Laouar2, Charles Bouchard2, Janet A. W. Elliott3,4, Nadr Jomha2
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1Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada
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2Department of Surgery, University of Alberta, Edmonton, AB, Canada
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3Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
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4Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada
11:40 – 12:00 Improving the post-thaw outcome of cryopreserved mesenchymal stem cells and peripheral blood mononuclear cells using ice recrystallization inhibitors
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Leah E. McMunn1 S. Khan2,3, J. Poisson1, L. Davila2, C. Mitz2, D.W. Courtman2,3, R.N. Ben1
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1Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada
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2Regenerative Medicine, Ottawa Hospital Research Institute, Ottawa, ON, Canada
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3Department of Medicine, University of Ottawa, Ottawa, ON, Canada
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12:00 – 1:00 Lunch (Provided)
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1:00 – 2:00 Keynote Session 2: Nanowarming for Regenerative Medicine
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John Bischof1,2
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1Department of Biomedical Engineering, University of Minnesota, MN, United States
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2Department of Mechanical Engineering, University of Minnesota, MN, United States
2:00 – 2:30 The Canadian Ice Core Lab
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Alison Criscitiello
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Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, AB, Canada
2:30 – 2:50 Hydraulic Parameters Obtained from Kinetic Cell Volume Measurements of HUVECs Fitted to a Model Assuming Non-Ideal Thermodynamic Behaviour
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Laura Gabler Pizarro1 Locksley E. McGann2, Janet A. W. Elliott1,2
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1Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada
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2Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
2:50 – 3:10 Modulating Rates of Intracellular Ice Recrystallization Using Permeating and Non-Permeating CPAs
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Nishaka William1 Jason P. Acker1,2
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1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
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2Centre for Innovation, Canadian Blood Services, Edmonton, AB, Canada
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3:10 – 3:30 Coffee Break (Provided)
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3:30 – 3:50 Overcoming solubility limitations with a new class of ice recrystallization inhibitors
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Anna Ampaw Robert N. Ben
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Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada
3:50 – 4:10 Rapid cool high-pressure cryopreservation
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Mohammad Amini James Benson
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Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada
4:10 – 4:30 Positive effect of 3-O-Methylglycose on post-thaw in bull sperm
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Maajid Bhat James Benson
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Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada
4:30 – 5:00 What’s Hot in the Cold Sciences – an Update
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Jason Acker1,2
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1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada
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2Centre for Innovation, Canadian Blood Services, Edmonton, AB, Canada
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5:30 Banquet (Faculty Club)
8:00 Ice Castles at Hawrelak Park